SKM 2023 – wissenschaftliches Programm

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DY: Fachverband Dynamik und Statistische Physik

DY 52: Statistical Physics of Biological Systems II (joint session BP/DY)

DY 52.4: Vortrag

Freitag, 31. März 2023, 10:15–10:30, BAR Schö

A stereotypical sequence of condensation and dispersal of RNA polymerase II clusters during stem cell differentiation — •Tim Klingberg1, Irina Wachter2, Agnieszka Pancholi2, Roshan Prizak2, Priya Kumar3, Yomna Gohar3, Marcel Sobucki2, Elisa Kämmer2, Süheyla Eroğlu-Kayikçi2, Sylvia Erhardt2, Carmelo Ferrai3, Vasily Zaburdaev1, and Lennart Hilbert21Friedrich-Alexander-Universität Erlangen-Nürnberg — 2Karlsruher Institut für Technologie — 3Universitätsmedizin Göttingen

Most eukaryotic genes are transcribed by RNA polymerase II (Pol II). In stem cells, recruited Pol II forms prominent, long-lived clusters, which gradually disappear during differentiation, so that only smaller clusters remain. Here, we ask whether the loss of large Pol II clusters is a stereotypical transition that can be explained by changes in the Pol II transcriptional state during differentiation. We assess clusters by super-resolution microscopy in three different experimental models of differentiation. In all cases, Pol II clusters first become larger and rounder, then unfold, and finally split into small clusters. These shape changes are accompanied by changes of transcriptional activity of Pol II. Previous work suggests a surface-condensate model, where enhancer regions support Pol II cluster formation, and transcriptional activity disperses clusters. Using this theoretical model, we propose that the developmental changes in enhancer marks and transcriptional activity during differentiation are sufficient to define a stereotyped trajectory through a cluster shape space.

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