SKM 2023 – wissenschaftliches Programm
DY 53.4: Vortrag
Freitag, 31. März 2023, 10:30–10:45, TOE 317
Optimal collective durotaxis through active wetting — Macià-Esteve Pallarès1, Irina Pi-Jaumà2, Isabela Corina Fortunato1, Valeria Grazu3, Manuel Gómez-González1, Pere Roca-Cusachs1, Jesus de la Fuente3, •Ricard Alert4, Raimon Sunyer1, Jaume Casademunt2, and Xavier Trepat1 — 1Institute for Bioengineering of Catalonia — 2University of Barcelona — 3Instituto de Nanociencia y Materiales de Argón — 4Max Planck Institute for the Physics of Complex Systems
The directed migration of cell clusters enables morphogenesis, wound healing and collective cancer invasion. Gradients of substrate stiffness are known to direct migration of cell clusters in a process called collective durotaxis, but underlying mechanisms remain unclear. Combining theory and experiments, we reveal a connection between collective durotaxis and the wetting properties of cell clusters. Our experiments show that durotaxis is non-monotonic with substrate stiffness, being optimal at intermediate stiffness. Modeling the cell clusters as active droplets, we explain this non-monotonic durotaxis in terms of a balance between active traction, tissue contractility, and surface tension. Finally, we show that the distribution of cluster displacements has a heavy tail, with infrequent but large cellular hops that contribute to durotactic migration. Our study demonstrates a physical mechanism of collective durotaxis based on the wetting properties of active droplets.