SKM 2023 – scientific programme
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KFM: Fachverband Kristalline Festkörper und deren Mikrostruktur
KFM 8: Diamond and related dielectric materials
KFM 8.5: Talk
Wednesday, March 29, 2023, 15:20–15:40, POT 51
Enhanced protein immobilization by nanostructuring of UNCD surface — •Daniel Merker1, Daniela Bertinetti2, Rolf Merz3, Michael Kopnarski3, Friedrich W. Herberg2, Johann P. Reithmaier1, and Cyril Popov1 — 1Institute of Nanostructure Technologies and Analytics (INA), Center for Interdisciplinary Nanostructure Science and Technology (CINSaT), University of Kassel, Germany — 2Department of Biochemistry, CINSaT, University of Kassel, Germany — 3Institut für Oberflächen- und Schichtanalytik GmbH, Kaiserslautern, Germany
Investigations of molecular mechanisms related to the formation of the inner clock require detection of coupling factors, e.g. secreted neuropeptides, that mediate synchronization. In this work we study the application of ultrananocrystalline diamond (UNCD) as biosensor for detection of such coupling factors. The crucial part is the feasibility of protein immobilization on UNCD surfaces. From all investigated coupling routs only photochemical attachment of alkenes gave acceptable results when the green fluorescent protein (GFP) was immobilized. A process to create nanostructures was developed to increase the effective surface for protein immobilization. Both functionalization and structuring have beneficial effects on immobilization performance, but especially in combination the immobilization efficacy increases significantly. Lastly, the immobilization of different binding proteins against GFP - antibodies, nanobodies, DARPins - was investigated, with the last two showing promising results for high specificity and affinity capture of target molecules in a future UNCD biosensor.