Berlin 2024 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 8: Poster Session Ia
BP 8.2: Poster
Montag, 18. März 2024, 18:00–20:30, Poster C
Imaging F-Actin arrangement via homo-FRET using 2D polarization fluorescence microscopy — Lukas Spantzel1,2, Chen Sun3, Lena Jesse1,2, Yunhao Mei1,4, Yutong Wang1,4, Shangjun Cheng1,2,4, Mohammad Soltaninezhad1,4, Michael Börsch1,2, Rainer Heintzmann1,4, Ivan G. Scheblykin3, Adrian T. Press1,2, and •Daniela Täuber1,4 — 1Friedrich Schiller University, Jena — 2University Hospital Jena — 3Lund University, Sweden — 4Leibniz Institute of Photonic Technology, Jena, Germany
Polymicrobial infection affects the organization of F-Actin in the cytoskeleton and the cortex causing cell death. We use 2-dimensional polarization fluorescence imaging (2DPOLIM) to visualize the aggregation of phalloidin-dye labeled F-Actin via Förster Resonance Energy Transfer (homo-FRET). The homo-FRET efficiency observed from fibrillar structures in mouse embryonal fibroblasts agrees well with that of single fibrillar F-Actin synthesized from non-muscle Actin. Higher values are observed from other structures inside the cells, representing a more dense aggregation of the F-Actin in those regions.
Keywords: F-Actin; homo-FRET; fluorescence polarization; cytoskeleton