Dresden 2026 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 14: Poster Session II
BP 14.33: Poster
Dienstag, 10. März 2026, 18:00–21:00, P2
Time resolved fluorescence anisotropy of single phalloidin-dye complexed F-Actin fibrils: MD simulation and experiments. — Phillip Seeber1, •Shangjun Cheng1,2, Lukas Spantzel1,3, Rainer Heintzmann1,2, and Daniela Täuber1,2 — 1Friedrich Schiller University Jena — 2Leibniz Institute of Photonic Technology, Jena — 3Jena University Hospital, Jena
Phalloidin conjugates are widely used to visualize actin filaments (F-Actin) due to their high binding affinity [1]. Fluorescence polarization imaging adds information on cellular structures known from other approaches [2, 3]. Moreover, super-resolution techniques such as STED and SIM often employ polarization optics. For aligned samples, such as actin filaments, insights in fluorophore orientation and wobbling dynamics will improve the interpretation of experimental results. Here, we apply quantum chemical methods including DFT, TD-DFT and ADC(2) to study the trajectory of the dye*s transition dipole moments during thermal fluctuations. We correlate the MD simulations with fluorescence anisotropy measurements acquired using time-resolved fluorescence lifetime imaging (FLIM) and 2D polarization fluorescence imaging (2D-POLIM) [3]. [1] Melak M., Plessner M., et al. Journal of cell science, 2017, 130, 525. [2] Rimoli, C. V., Valades-Cruz C.A., et al. Nat Commun 2022, 13, 301. [3] Camacho R., Täuber D., et al. Communications Biology, 2018, 1, 157.
Keywords: Polarization Imaging; F-Actin; Phalloidin conjugates; MD simulation; Time-resolved fluorescence lifetime imaging