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BP: Fachverband Biologische Physik
BP 14: Poster Session II
BP 14.37: Poster
Dienstag, 10. März 2026, 18:00–21:00, P2
Implementation of NIR excitation and detection in 2D-POLIM setup — Shangjun Cheng1,2, •Zilong Huang1, Rainer Heintzmann1,2, and Daniela Täuber1,2 — 1Friedrich Schiller University Jena — 2Leibniz Institute of Photonic Technology, Jena
Two-dimensional polarization fluorescence imaging (2D-POLIM) has been used to probe the orientation/rotation of molecules and the Förster resonance energy transfer between closely located chromophores [Camacho et al., Adv. Mat. 2019, 31, 1805671; Camacho et al., Commun. Biol. 2018, 1, 157]. 2D-POLIM can provide full in-plane information on the polarization state of the sample through synchronized control of the excitation and detection polarizations. Near-infrared (NIR) excitation reduces the background from intrinsic autofluorescence in cells and tissue samples. Here, we show a stable, precisely controllable excitation polarization of a 785 nm laser. We also demonstrate the potential of 2D-POLIM in imaging and analysis of molecular orientation in the NIR range.
Keywords: Polarization; Orientation; Energy transfer; NIR excitation