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BP: Fachverband Biologische Physik

BP 14: Poster Session II

BP 14.42: Poster

Dienstag, 10. März 2026, 18:00–21:00, P2

Visualizing Immune Cell Behaviour in 3D: An Ex Vivo Lattice Lighsheet Microscopy and Analysis Framework — •Anna Schepers¹, Joannah Fergusson¹, Edward Wheeler¹, Jacky Ko¹, Robert Kochl², and Marco Fritzsche¹ — ¹Kennedy Institute of Rheumatology, University of Oxford, UK — ²King's College London, UK

The inherently multiscale immune response is regulated by diverse cell interactions, relying on cues from tissues down to single cells and subcellular structures. The intricate dynamics of the immune system present challenges for the observation of the immune response. A technological advance has been achieved with the introduction of lattice light sheet microscopy (LLSM), allowing fast and gentle imaging of live samples while achieving subcellular resolution. By complementing LLSM-based volumetric imaging with advanced sample handling of ex vivo tissue samples and perfusion imaging chambers, we provide a system that preserves critical physiological complexity. We present a complex data processing and analysis framework for robust 3D segmentation and tracking cells in the complex tissue environment allowing cell profiling from live cell behaviour. We show that in our setup, we can follow single cells and their interactions in volumes several cell layers deep in living samples within their environment, providing nuanced insights into the immune response.

Keywords: immune cells; cell interactions; lattice light sheet microscopy; 4D microscopy