Dresden 2026 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 14: Poster Session II
BP 14.45: Poster
Dienstag, 10. März 2026, 18:00–21:00, P2
Realignment and realization of polarization-resolved confocal FLIM — •Lizhong Mou1, Shangjun Cheng1,2, Subham Adak1,2, Maryam Ali1,2, Daniela Täuber1,2, and Rainer Heintzmann1,2 — 1Friedrich Schiller University Jena — 2Leibniz Institute of Photonic Technology, Jena
Fluorescence lifetime imaging microscopy (FLIM) is an attractive technique in the life sciences that enables quantitative mapping of excited-state lifetimes in the regime of nanoseconds within microscopic images [Le Marois et al., J. Biophot. 2017, 10,1124]. Conventional fluorescence polarization is widely used to assess the orientation and rotation of molecules but typically relies on millisecond acquisition times [Camacho et al., Adv. Mat. 2019, 31, 1805671]. Here, we present an alignment protocol for polarization-resolved confocal FLIM that delivers nanosecond temporal resolution and high-quality imaging. We further investigate time-resolved polarization states of dye molecules from cell and tissue samples.
Keywords: Bioimaging; FLIM; Polarization