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BP: Fachverband Biologische Physik

BP 14: Poster Session II

BP 14.77: Poster

Dienstag, 10. März 2026, 18:00–21:00, P2

Investigating the binding of clients to small heat shock protein 16.5 using electrospray ion bean deposition and cryogenic electron microscopy — •Manami Imada, Noor Naseeb, and Stephan Rauschenbach — University of Oxford, Oxford, UK

Small heat shock proteins (sHSP) act as the first line of defence, preventing protein aggregation. sHSP do this by binding to the protein under stress to form a stable complex. We explore how these sHSPs bind to clients such as lysozymes using cryogenic electron microscopy (cryo-EM). However, sHSP are hard to image due to their dynamic nature and potential to form different oligomers.

Electrospray ion bean deposition (ES-IBD) is used to deposit the sample onto the cryo-EM grids. ES-IBD utilises native mass spectrometry (nMS), which transfers the complexes into the gas phase intactly and allows for mass filtration of the ion beam by a quadrupole. This allows specific complexes and binding stoichiometries to be deposited selectively onto the cryo-EM grids to be imaged.

Keywords: Cryo-EM; ES-IBD; native mass spectrometry; heat shock proteins