Dresden 2026 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 26: Biomaterials and Biopolymers (joint session BP/CPP)
BP 26.8: Vortrag
Donnerstag, 12. März 2026, 11:45–12:00, BAR/SCHÖ
Controlling Axonal Outgrowth of Organoids by 3D Nanoprinted Scaffolds — •Tobias Müller1, Malte Siegmund1, Emma Wollesen1, Kim Krieg2, Ole Pless2, Jan Hahn3, Robert Zierold1, and Robert Blick1 — 1Center for Hybrid Nanostructures, University of Hamburg, 22761 Germany — 2Fraunhofer ITMP, Discovery Research Screening Port, 22525 Germany — 3Section Facility Mass Spectrometry and Proteomics, University Medical Center Hamburg-Eppendorf, 20246 Germany
Cortical organoids are promising models for neurodegenerative disease research, yet their integration into defined neural networks remains challenging. We use two-photon polymerization (2PP) to fabricate three-dimensional scaffolds that direct axonal outgrowth and support organoid integration into engineered circuits.
Scaling structures from single neurons to millimeter-scale organoids introduces adhesion and imaging challenges. We address this by combining tailored scaffold geometries with a polydimethylsiloxane (PDMS)-based anchoring strategy, critical-point drying and quantitative analysis of electron microscopy images. Walls, stairs and microwires enhance organoid-scaffold interactions, promoting axonal outgrowth along intended paths and reducing extension into non-target regions.
These results demonstrate that 2PP-fabricated microarchitectures can guide axon growth and stabilize organoid-substrate contact, enabling more controlled organoid-based neuronal networks and advancing brain-on-a-chip approaches.
Keywords: 3D nanoprinting; Cortical organoids; guided neuronal networks; 3D cell culture structures; brain-on-a-chip