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BP: Fachverband Biologische Physik
BP 33: Bioimaging
BP 33.10: Vortrag
Donnerstag, 12. März 2026, 17:45–18:00, BAR/0205
Imaging biomolecules for improving single-molecule diffraction — •Stefanie Lenzen1,2, Lukas V. Haas1,2, Kevin Janson1, Amit K. Samanta1,2, and Jochen Küpper1,2 — 1Center for Free-Electron Laser Science (CFEL), Deutsches Elektronen-Synchrotron DESY, Hamburg — 2Department of Physics & Department of Chemistry & Center for Ultrafast Imaging, Universität Hamburg
Determining the structure and dynamics of single native biomolecules is still a challenge. In protein-crystallography and cryo-EM the molecule needs to be fixed, which might lead to structural disentegration, and the temporal resolution of these methods are limited. X-ray free-electron lasers (XFELs) provide ultrashort pulses, enabling diffraction before destruction, and a large number of photons, promising the observation of diffraction patterns off single nanoparticles [1]. Aerodynamic-lens stacks were used to deliver focused dense particle beams for such experiments on large nanoparticles [2]. Localization microscopy (LM), based on Mie-scattering is used to study and optimize these beams, an important step in improving single particle imaging. Due to the limitation in particle size [3], small biomolecules do not provide sufficient intensity for being detected with LM. We optimized the optical and analysis system and developed a promising method for the detection of smaller biomolecules, based on fluorescence.
[1] Poudyal, Schmidt, Schwander, Struct. Dyn. 7, 024102 (2020);
[2] Ayyer, et int (39 authors), Chapman, Optica 8, 15-23 (2021);
[3] Worbs, et int (2 authors), Maia, Commun Phys 8, 155 (2025)
Keywords: single particle imaging; optical scattering microscopy; proteins; fluorescence