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BP: Fachverband Biologische Physik

BP 33: Bioimaging

BP 33.2: Vortrag

Donnerstag, 12. März 2026, 15:30–15:45, BAR/0205

Using Rotating Coherent Scattering (ROCS) Microscopy for Binding and Uptake Analysis of Virus-Mimicking Particles — •Dominik Huber and Alexander Rohrbach — Lab for Bio- and Nano-Photonics, University of Freiburg, Germany

The investigation of viruses with their host cell is an important topic in medical research, yet direct observation of these nanoscale dynamics remains challenging, since conventional fluorescence microscopy is limited by photobleaching and labelling constraints. To address these limitations, we employ label-free Rotating Coherent Scattering (ROCS) microscopy, an imaging approach that enables high-speed, high-resolution visualization of virus-mimicking particle behaviour at the cell.

ROCS microscopy uses backscattering of oblique illumination of a rotating laser beam to achieve around 160 nm spatial resolution at imaging rates up to around 200 Hz. By combining several illumination wavelengths and illumination angles, ROCS microscopy can be used in brightfield and darkfield as well as in total internal reflection mode. Leveraging this flexibility in imaging, allows us to capture the binding dynamics of particles to cells and to investigate them from their first attachment up to the investigation of single particle uptake events.

The analysis of particle fluctuation widths and mean square displacements enabled us to detect differences in binding characteristics of two different types of virus-mimicking particles, thus demonstrating that ROCS microscopy provides a powerful tool for investigation of particle-cell interactions at the single-particle level.

Keywords: Rotating Coherent Scattering (ROCS) Microscopy; Virus-cell interactions; Label-free imaging; Single-particle tracking