Dresden 2026 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 33: Bioimaging

BP 33.3: Vortrag

Donnerstag, 12. März 2026, 15:45–16:00, BAR/0205

Pharmacological modulation of intrinsic tissue transparency for enhanced microscopy — •Adrián Puerta, Susan Wagner, and Moritz Kreysing — Institute of Biological and Chemical Systems, Karlsruhe Institute of Technology, 76344 Eggenstein Leopoldshafen, Germany

Light microscopy remains as one of the primary methods for data acquisition in biological research, yet its performance is frequently limited by the strong scattering properties of experimental tissues. Hence, the use of clearing agents that are incompatible with living samples is often required (1). To overcome these limitations, we aim to modulate the intrinsic transparency of biological samples, enabling more effective live-cell imaging.

Conventional clearing strategies rely on chemicals that modify the refractive index of aqueous or lipid-rich tissue components (2). In contrast, our approach involves screening small molecules with pharmacological properties that may enhance transparency in mammalian cells. To investigate this, we have developed a high-throughput screening system based on flow cytometry that will allow us to identify potential changes at a single-cell scale.

(1) Yu, T. et al. Physical and chemical mechanisms of tissue optical clearing. iScience 24, 102178 (2021).

(2) Ou, Z. et al. Achieving optical transparency in live animals with absorbing molecules. Science 385, eadm6869 (2024).

Keywords: Optical Microscopy; High-Throughput Screening; Tissue Transparency