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BP: Fachverband Biologische Physik
BP 37: Tissue Mechanics II
BP 37.3: Vortrag
Freitag, 13. März 2026, 10:00–10:15, BAR/0106
Holographic vibration spectroscopy - extracting mechanical properties of adherent cells from their vibrational response — •Eric Schneider1, Bob Fregin1, Dominic Mokbel2, Sebastian Aland2, and Oliver Otto1 — 1Institute of Physics, University of Greifswald, Greifswald, Germany — 2Institute of Numerical Mathematics and Optimization, TU Bergakademie Freiberg, Freiberg, Germany
The mechanical properties of biological cells are closely linked to their pathophysiological state, and high-throughput quantification is essential for using them as biomarkers in basic and translational research. While microfluidic technologies can characterize suspended cells at rates above 1,000 cells per second, no comparable method exists for adherent cells or tissues. Here, based on preliminary experiments with holographic vibration spectroscopy (HVS), developed in our group, we present a theoretical framework to address this gap. In HVS, adherent cells are harmonically oscillated at defined frequencies and amplitudes, and their deformation and phase shift encode their mechanical properties. To validate this concept, we developed a finite-element simulation framework using the open-source library AMDIS, capable of modeling cellular vibration across a broad parameter space. From these simulations, we derive a protocol to solve the inverse problem and show that viscoelastic properties can be uniquely determined using only the amplitude response at two vibration frequencies. Combined with HVS, this framework will next be used to rapidly and non-invasively quantify the viscoelastic properties of adherent cells and tissues.
Keywords: Cell mechanics; Finite-element simulation; Adherent cells; Tissue mechanics; High-throughput analysis