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BP: Fachverband Biologische Physik

BP 5: Membranes, Vesicles and Synthetic Life-like Systems I

BP 5.3: Vortrag

Montag, 9. März 2026, 15:30–15:45, BAR/0205

Supported DPPC/DPPG Bilayers on Oxide Substrates as a Versatile Platform for Protein*Membrane Studies and Future FRET-Based Sensing — •Daniel Saavedra¹, Benjamin Ruz¹, Marcelo Cisternas², Susana Rojas², and Ulrich Volkmann¹ — ¹Institute of Physics, Pontificia Universidad Católica de Chile, Santiago, Chile — ²School of Industrial Engineering, University of Valparaíso, Santiago, Chile

We develop a dry-processed supported lipid platform to study how membrane composition and substrate chemistry modulate protein-lipid interactions. Following the dry two-step self-assembly method for DPPC on silicon [1], we prepare DPPC bilayers with gramicidin A on SiO2 and characterize them by temperature ramps using very-high-resolution ellipsometry, AFM, FTIR, and SERS. Ellipsometry resolves DPPC pre- and main-phase transitions and peptide-induced shifts in thermal stability, which correlate with AFM domain morphology and FTIR amide I changes. In parallel, DPPC/DPPG bilayers are deposited on SiO2 and TiO2 and probed by AFM, FTIR, and ellipsometry to disentangle the influence of lipid charge and oxide surface on bilayer continuity and phase behavior, consistent with previous studies on lipid oxide interfaces [2]. Together, these results establish a solvent-free, thermally robust lipid architecture compatible with multimodal read-out and future integration of carbon quantum dots for FRET-based sensing. Acknowledgements: ANID Fellowship (DS) References 1.Cisternas MA et al. Int J Mol Sci. 2020;21:6819. 2.Tero R et al. Proc SPIE. 2007;6769:67690J.

Keywords: Supported lipid bilayer; DPPC/DPPG membranes; Protein–lipid interactions; Biosensor; Potencial surfaces interactions