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BP: Fachverband Biologische Physik
BP 7: Poster Session I
BP 7.28: Poster
Montag, 9. März 2026, 15:00–17:00, P5
Deciphering the role of flagellar membrane glycoproteins in ciliary adhesion of Chlamydomonas reinhardtii — •Lea Rupprecht1, Adrian Nievergelt2, Rodrigo Catalan1, Lara Hoepfner3, and Oliver Bäumchen1 — 1University of Bayreuth, Experimental Physics V, 95447 Bayreuth, Germany — 2Max Planck Institute of Molecular Plant Physiology, 14476 Potsdam, Germany — 3Human Technopole, 20157 Milan, Italy
Elucidating the physical mechanisms of microbe-surface interactions is essential for developing novel technologies to control the formation of microbial biofilms. While most studies focus on bacteria as model systems, the adhesion of eukaryotic photosynthetic microorganisms to surfaces remains rather elusive. Chlamydomonas reinhardtii has been shown to adhere to surfaces with its two cilia under blue light [1], yet the underlying molecular mechanism remains unclear. For decades, the N-glycosylated proteins FMG1-B were considered the main adhesive components of the ciliary glycocalyx, with FMG1-A also contributing to its organization and function. We performed in vivo single-cell micropipette force spectroscopy [2] and adsorption/desorption [3] experiments on CRISPR/Cas9-generated FMG1-B-, FMG1-A-, and double-knockouts of C. reinhardtii. Thereby we examine how the absence of specific glycocalyx components affects ciliary adhesion forces in different light conditions.
[1] Kreis et al., Nature Physics 14, 45-49 (2018).
[2] Backholm and Bäumchen, Nature Protocols 14, 594-615 (2019).
[3] Catalan et al., Soft Matter 19, 306-314 (2023).
Keywords: Chlamydomonas; Cell adhesion; Cilia