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BP: Fachverband Biologische Physik
BP 7: Poster Session I
BP 7.31: Poster
Montag, 9. März 2026, 15:00–17:00, P5
Passive microrheology reveals anisotropic intracellular activity — •Sarah Louisa Lädke1, Till Moritz Münker1, Matthias Krüger2, and Timo Betz1 — 1Third Institute of Physics - Biophysics, Georg August Universität Göttingen — 2Institute for Theoretical Physics, Georg August Universität Göttingen
To investigate intracellular activity and the associated deviations from thermodynamic equilibrium, we study the fluctuations of endogenous vesicles and phagocytosed beads in various cell types and conditions. Experimentally, we combine darkfield microscopy with high-speed imaging and advanced image post-processing techniques, enabling the acquisition of trajectories with spatial and temporal resolution in the order of nanometers and milliseconds, respectively. We apply a novel observable, termed Mean Back Relaxation (MBR) [Münker et al., Nature Materials, 2024], to these trajectories. The MBR quantifies non-equilibrium in confined systems and establishes a link between intracellular particle fluctuations and their effective energies. In doing so, we aim to extend the principles of passive microrheology to non-equilibrium environments. The MBR derived from our trajectories breaks time-reversal symmetry and exhibits pronounced anisotropies, indicating not only heterogeneous cellular structures but also anisotropic activity.
Keywords: Intracellular activity; Mean Back Relaxation; Passive microrheology