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BP: Fachverband Biologische Physik
BP 7: Poster Session I
BP 7.43: Poster
Montag, 9. März 2026, 15:00–17:00, P5
Structural changes of sarcomeres in iPSC-derived 3D engineered skeletal muscle after laser-induced injury — •Lisa-Marie Scharfenstein1,2, Daniel Härtter1, Mahboubeh Farajian2, Mattias Luber2, Timo Betz2, Wolfram Zimmermann1, and Arne Hofemeier1 — 1Department of Pharmacology and Toxicology, University Medical Center Göttingen, Göttingen, Germany — 2Third Institute of Physics, University of Göttingen, Göttingen, Germany
Skeletal muscle injury models are employed to study mechanisms of muscle repair and regeneration. Commonly used muscle injury models such as cryo-injury, crush-injury and cardiotoxin-treatment do not allow for precisely localized muscle damage. Here, we introduce precise laser-based injury in human iPSC-derived 3D engineered skeletal muscle (ESM) tissue grown in a custom-made culture platform. Using high-resolution live microscopy and a sarcomere reporter model (ACTN2-Citrine), we monitor Z-band morphology on timescales of seconds to several days using the previously by our group introduced AI-based Sarcomere Analysis Multi-tool (SarcAsM). In doing so, we find an average sarcomere elongation of (0.45 +- 0.061) mum (mean +- sem) within 50 mum distance from the injury, that further decays exponentially from the location of injury towards the periphery, suggesting local impaired tensional homeostasis in myofibers surrounding the injury. Taken together, we report first data obtained in a novel human iPSC-based skeletal muscle injury model, which we intend to use for repair and regeneration inducing drugs.
Keywords: Sarcomeres; Laser-ablation; Skeletal muscle injury model