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BP: Fachverband Biologische Physik

BP 7: Poster Session I

BP 7.9: Poster

Montag, 9. März 2026, 15:00–17:00, P5

Controlled Spray coating of Bacterial Nanocellulose Thin films revealed by Surface-Sensitive X-ray Scattering — •Joanne Neumann1, LI Li2, Edina Klein1,3, Jan Rubeck1, Ute Römling2, Holger Sonderrmann1,3, and Matthias Schwartzkopf11DESY, Photon Science, Notkestr. 85, D-22607 Hamburg — 2Department of Microbiology, Tumor and Cell Biology, Biomedicum, KI, Karolinska Institutet, Nobels väg 6, S-17177 Stockholm — 3CSSB, Center for Structural Systems Biology, Notkestr. 85, D-22607 Hamburg

Bacterial nanocellulose (BNC) is a sustainable biomaterial valued for its purity, nanoscale fibrillar structure, and biocompatibility. Its properties can be tailored through genetic engineering, growth conditions, and processing. Here, we fabricate thin films from BNC produced by S. typhimurium, Komagataeibacter, and Klebsiella using airbrush spray deposition of aqueous dispersions as a green, reproducible method. We examine how different extraction procedures (acidic, alkaline, surfactant-based) affect nanoscale organization and film adaptability. High-resolution X-ray scattering (µGIUSAXS/µGIWAXS) is used to probe fibril orientation, aggregation, and hierarchical ordering. Our results show extraction-dependent structural variations that influence film homogeneity and functional performance. By refining spray-coating and scattering protocols, this work links chemical treatment history to structural adaptability and supports sustainable strategies for controlled BNC thin-film fabrication in materials science and bioengineering.

Keywords: Bacterial Nanocellulose; Sensitive X-ray Scattering; Airbrush Spray Deposition; Sustainable Biomaterial; Thin Film

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