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O: Fachverband Oberflächenphysik

O 19: Vacuum Science & Technology: Theory and Applications – Poster

O 19.3: Poster

Montag, 9. März 2026, 18:00–20:00, P2

Electrospray Ion Beam Deposition for Atomic Resolution Native Protein Structure Investigation at Surfaces — •Stephan Rauschenbach — University of Oxford, Kavli Institute for Nanoscience Discovery, Department of Chemistry, Sherringdon Road, Oxford

Electrospray ion beam deposition (ESIBD) enables controlled deposition of intact biomolecules from solution onto surfaces under ultrahigh vacuum conditions. We present ESIBD as a sample preparation method for cryo-EM, scanning probe, and holography applications, bridging native electrospray ionisation mass spectrometry with surface-based structural characterisation techniques.

The method combines mass-selective soft landing with controlled ice growth embedding protocols. Proteins maintain their native conformations during the gentle electrospray process and deposition. For cryoEM Ice matrix formation occurs post-deposition minimising mechanical stress and denaturation.

Here we discuss the state of the art implementation of this method on commercial and self-built platforms and demonstrate atomic resolution imaging of soluble and membrane proteins deposited on surfaces in vacuum.

Keywords: electrospray ion beam deposition; electron microscopy; protein