Dresden 2003 – wissenschaftliches Programm

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CPP: Chemische Physik und Polymerphysik

CPP 3: Biomoleküle

CPP 3.1: Vortrag

Montag, 24. März 2003, 10:15–10:30, ZEU/118

Biomaterials in Microchannel Mixing Devices Manufactured by Soft Lithography Techniques — •Alexander Otten, Sarah Köster, Rainer Gauggel, Stephan Herminghaus, and Thomas Pfohl — Angewandte Physik, Universität Ulm, Albert-Einstein-Allee 11, D-89069 Ulm

Handling and manipulation of extremely small volumes are important issues whenever biomaterials are used, which are often hard to isolate in larger amounts. The usage of microfluidic devices does not only allow to analyze small volume fractions but also gives access to faster and more sensitive characterization. We use several kinds of microchannel systems such as crossed single channels, parallel aligned channels and curved channels with variable radii for the manipulation of biomaterials. The microchannels were manufactured by soft lithography and the experiments were done by using fluorescence microscopy and confocal Raman microscopy. In a crossed-channels device the laminar flow gives access to diffusional mixing experiments by hydrodynamically focusing a center stream by two side streams. The resulting stream profile is in a steady state and therefore different time slots in the evolution of the mixing reactions can be selected by varying the observation position along the stream. Using the Raman contrast imaging method, first complexation experiments of unlabeled DNA⁻ with Co³⁺ ions were carried out.