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DPG

Regensburg 2004 – wissenschaftliches Programm

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AKB: Biologische Physik

AKB 50: Poster Session "Biological Physics"

AKB 50.99: Poster

Freitag, 12. März 2004, 10:30–13:00, B

DNA translocation through a biological nanopore — •Ulrich F. Keyser1, N. Wennersbusch1, D. J. Bonthuis1, N. H. Dekker1, X. S. Ling2, and Cees Dekker11Molecular Biophysics, Delft University of Technology, Lorentzweg , 2628 CJ Delft, The Netherlands1 — 2Brown University, Providence, USA

We study the translocation of single-stranded DNA (ss-DNA) molecules through a single biological nanopore, viz., an alpha-Hemolysin pore from Staphylococcus aureus assembled within a lipid membrane. The inner diameter of this nanopore is only 1.5 nm and ss-DNA molecules can be transferred one by one. Applying a bias voltage at high salt concentrations gives rise to an ionic current through the nanopore. If a ss-DNA molecule enters the pore the current is partly blocked and the passage of a molecule is detected. We are investigating different types of ss-DNA with various lengths and sequences to study the influence on the translocation speed and current. The experimental results will be compared to recent theoretical models.

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