Regensburg 2004 – wissenschaftliches Programm

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SYLS: Life Sciences on the Nanometer Scale - Physics Meets Biology

SYLS 3: Symposium "Life Sciences on the Nanometer Scale - Physics Meets Biology"

SYLS 3.36: Poster

Mittwoch, 10. März 2004, 16:00–18:30, B

UV-Laser Induced Fluorescence Detection of Proteins in PDMS Microfluidic Devices — •Wibke Hellmich, Alexandra Ros, and Dario Anselmetti — Experimental Biophysics, Physics Department, Bielefeld University, Bielefeld, Germany

Since the complete mapping of the human genome growing interest in the field of proteomics requires new techniques for protein analysis. Especially efficient and sensitive methods for separation, detection and analysis of unlabeled protein solutions are of great importance. This work focuses on the separation and label free fluorescence detection of proteins in the UV range in uncoated polydimethylsiloxane (PDMS) microfluidic devices.

In the visible range we determined a detection limit of 100fM for fluorescein, a fluorescent dye, and in the UV-range 50µ M for tryptophane (Trp), an aromatic amino acid. In a first test experiment the separation of a protein mixture, avidin (four Trp) and lysocyme C (six Trp), in a microfluidic system with UV detection was successfully realized.

Further investigation will include the optimization of the detection system to enhance the sensitivity of this label free protein analysis method.