Regensburg 2004 – wissenschaftliches Programm

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SYLS: Life Sciences on the Nanometer Scale - Physics Meets Biology

SYLS 3: Symposium "Life Sciences on the Nanometer Scale - Physics Meets Biology"

SYLS 3.9: Poster

Mittwoch, 10. März 2004, 16:00–18:30, B

Direct Observation of Tiers in the Energy Landscape of a Chromoprotein: A Single-Molecule Study — •Martin Richter¹, C. Hofmann¹, T.J. Aartsma², H. Michel³, and J. Köhler¹ — ¹Experimental Physics IV, University of Bayreuth — ²Department of Biophysics, Leiden University — ³Department of Molecular Membrane Biology, Max-Planck Institute of Biophysics, Frankfurt

We report the observation of spectral fluctuations in the B800 band of light harvesting 2 (LH2) complexes from Rhodospirillium molischianum. The electronically excited states of these chromophores are mainly localized on individual BChl a molecules and can serve as local probes to monitor changes in the protein envirornment. The data provide information about the organization of the energy landscape of the protein in tiers that can be characterized by an average barrier height. In addition a clear correlation for the transition rates between those states and the energy separation of the levels involved is uncovered.