Dresden 2006 – wissenschaftliches Programm

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AKB: Biologische Physik

AKB 15: Biopolymers II

AKB 15.1: Vortrag

Dienstag, 28. März 2006, 16:30–16:45, ZEU 260

Apparent Persistence Length Renormalization of Bent DNA — •Helmut Schiessel¹, Igor M. Kulic², Herve Mohrbach³, and Rochish Thaokar⁴ — ¹Instituut-Lorentz, Universiteit Leiden, The Netherlands — ²Dept. of Physics and Astronomy, University of Pennsylvania, Philadelphia, USA — ³LPMC, Metz University, France — ⁴Dept. of Chemical Engineering, IIT, Bombay, India

We derive the single molecule equation of state (force-extension relation) for DNA molecules bearing sliding loops and deflection defects. Analytical results are obtained in the large force limit by employing an analogy with instantons in quantum mechanical tunneling problems. The results reveal a remarkable feature of sliding loops - an apparent strong reduction of the persistence length. We generalize these results to several other experimentally interesting situations ranging from rigid DNA-protein loops to the problem of anchoring deflections in AFM stretching of semiflexible polymers. Expressions relating the force-extension measurements to the underlying loop/boundary deflection geometry are provided and applied to the case of the GalR-loop complex.