Dresden 2006 – wissenschaftliches Programm
Bereiche | Tage | Auswahl | Suche | Downloads | Hilfe
AKB: Biologische Physik
AKB 22: Sensory Biophysics and Signal Transduction
AKB 22.6: Vortrag
Donnerstag, 30. März 2006, 16:00–16:15, ZEU 255
PROCEEDINGS IN ODORANT RECEPTOR EXPRESSION: FROM CELLULAR SYSTEMS TOWARDS IN VITRO SYSTEMS — •Eva Sinner1,2, Rudolf Robelek1, Eva Lemker2, Birgit Wiltschi2, and Dieter Oesterhelt2 — 1MPI for Polymer Research , Ackermannweg 10, 55128 Mainz — 2MPI for Biochemistry, Am Klopferspitz 18a, 82152 Martinsried
An in vitro strategy is now available to generate a platform for investigation of complex membrane proteins, such as odorant receptors. Coding for a complex membrane protein, an odorant receptor OR5 was used as challenging example to be inserted in an artificial planar lipid membrane system. We show the presence and orientation of resulting OR5 protein in the planar lipid membrane by tag-specific immuno- labelling in combination with surface plasmon enhanced fluorescence spectroscopy. Integration of the OR5 proteins are shown by radioactive labelling and a proof of function (specific ligand binding) is shown by surface enhanced infrared spectroscopy.