Dresden 2006 – wissenschaftliches Programm

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AKB: Biologische Physik

AKB 30: Poster Session I

AKB 30.14: Poster

Montag, 27. März 2006, 15:30–18:00, P1

Spatially and spectrally resolved fluorescence correlation spectroscopy — •Markus Burkhardt and Petra Schwille — Biotec/ TU Dresden, Tatzberg 47-51, 01307 Dresden, Germany

Fluorescence Correlation Spectroscopy (FCS) is based on time dependent fluorescence intensity fluctuations of labeled biomolecules as they enter and leave a diffraction-limited optical detection volume. From simple autocorrelation analysis, concentrations, diffusion and binding coefficients are easily obtained. An accurate measure of inter- and intramolecular dynamics is attainable by evaluating the concomitant fluctuations of two or more spectrally distinct fluorophores.

In a standard FCS setup, all these information are collected from one specific focus position inside the sample. For the investigation of cellular mechanisms however, there is a great interest in large scale transport and flow properties which cannot directly be assessed.

The aim of the current project is the realization of a flexible multi-spot illumination and array detection platform. First proof of principle FCS measurements with spatially and spectrally resolved detection systems show the feasibility of such an approach.