Dresden 2006 – wissenschaftliches Programm
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AKB: Biologische Physik
AKB 30: Poster Session I
AKB 30.36: Poster
Montag, 27. März 2006, 15:30–18:00, P1
FORCE SPECTROSCOPY ON SINGLE INTEGRIN-INVASIN BONDS — •Agnieszka Ligezowska1,2, Kristian Boye1,3, Johannes Eble4, Bernd Hoffmann1, and Rudolf Merkel1 — 1Institute of Thin Films and Interfaces, Research Centre Juelich, 52425 Juelich, Germany — 2Jagiellonian University Cracow, Institute of Physics, Reymonta 4, 30-059 Cracow, Poland — 3University of Southern Denmark, Memphys Center for Biomembrane Physics, Campusvej 55, DK-5230 Odense M, Denmark — 4Institute for Physiological Chemistry, Muenster University Hospital, 48149 Muenster, Germany
Force-induced dissociation of single specific bonds is a stochastic process which has attracted considerable interest during the last decade.
We have investigated the force-induced dissociation of bonds between two integrins, α3β1 and α7β1, and the ligand invasin 497. These proteins were immobilized in their active form on melamine microbeads and the single-bond regime was reached by receptor blocking with free invasin. Piconewton forces were applied by osmotically swollen red blood cell which acted as an ultrasoft spring. This system allowed us to measure yield forces at specified bond loading rates and different transducer stiffnesses.