Dresden 2006 – wissenschaftliches Programm

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AKB: Biologische Physik

AKB 30: Poster Session I

AKB 30.4: Poster

Montag, 27. März 2006, 15:30–18:00, P1

Studying Slow Membrane Dynamics with Continuous Wave Scanning Fluorescence Correlation Spectroscopy — •Jonas Ries and Petra Schwille — TU Dresden, Biophysik, Tatzberg 47-51, 01307 Dresden

Two Photon Scanning Fluorescence Correlation Spectroscopy (SFCS) has been shown to be a useful technique to analyze the dynamics of model membranes (GUVs). Here we discuss the application of SFCS using continuous wave excitation. The improved countrate enables the study of very slow diffusion in model membranes and cells as well as parameter-free determination of diffusion constants using two foci fluorescence cross correlation spectroscopy. Two color fluorescence cross correlation spectroscopy with continuous or pulsed interleaved excitation (PIE) allows binding studies on membranes. Reduction of photobleaching, reproducibility and stability compared to traditional FCS on membranes and the simple implementation in a commercial microscopy setup make SFCS a valuable addition to the pool of fluorescence fluctuation techniques.