Dresden 2006 – wissenschaftliches Programm
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AKB: Biologische Physik
AKB 40: Poster Session II
AKB 40.48: Poster
Mittwoch, 29. März 2006, 16:30–19:30, P3
Multifunctional liposomes: controlled permeability and micromanipulation — •Yannic Ramaye1, Joana Gomes1, Tristan Ruysschaert2, Didier Fournier2, Jürgen Fritz1, and Mathias Winterhalter1 — 1International University Bremen, Germany — 2Institut Pharmacologie et Biologie Structurale, UMR5089, Toulouse, France
Enzymes are able to accelerate biochemical reactions by many orders of magnitudes and it is tempting to use them for biotechnological synthesis. However, instability, their low recovery during biocatalytic processes and their high cost make them commercially less advantageous. A possible method to protect proteins from hostile environment is via encapsulation in vesicles. We control the permeability of water soluble substance by reconstitution of natural or bio-engineered channel forming proteins. For example, Acetylcholinesterase is highly sensitive to pesticides and was engineered to become a tool for pesticide detection. We have shown that encapsulation into liposomes stabilizes the enzyme against dilution effect and protects it against proteolytic agents. High encapsulation yield was achieved using affinity to bind the free enzyme to the capsule surface. Vesicles with covalently bonded complementary strands were bound to the surface of a DNA-chip. This allows to functionalize a large number of capsules on specific areas. Magnetic liposomes are synthetized by swelling dried lipids with magnetic fluid based on maghemite citrated nanoparticles. The incorporation of these nanoparticles allows liposome manipulation by applying a magnetic field. To increase stabilization, vesicles can easily be coated with polyelectrolytes.