Dresden 2006 – wissenschaftliches Programm
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AKB: Biologische Physik
AKB 40: Poster Session II
AKB 40.69: Poster
Mittwoch, 29. März 2006, 16:30–19:30, P3
Diffusion along Microfluidic Channels — •Andreas Heeren1, Cheng-Ping Luo1, Guenter Roth2, Alexander Ganser3, Roland Brock3, Karl-Heinz Wiesmueller2, Wolfgang Henschel1, and Dieter Kern1 — 1Institute of Applied Physics, University of Tuebingen, 72076 Tuebingen, Germany — 2EMC microcollections GmbH, 72070 Tuebingen, Germany — 3Institute for Cell Biology, University of Tuebingen, 72076 Tuebingen, Germany
Living cells respond simultaneously to a variety of different stimuli. To achieve a specific cellular response a well defined mixture of stimuli or agents is required. However, in order to identify optimum mixtures of even few different compounds with respect to their relative and absolute concentrations, large numbers of biological tests are needed. Diffusion represents a highly efficient means for the generation of substance mixtures, namely continuously varying concentration profiles, with a minimum of pipetting steps. Here we present an array of microfluidic channels for the generation of binary substance mixtures with only two pipetting steps. For this purpose a microfluidic structure with a height of 500 µm was fabricated using the negative tone resist SU8. In a test diffusion of fluorescein dissolved in water was observed by fluorescence microscopy. The diffusion constant was determined by analyzing the fluorescence micrographs. Furthermore a procedure to detect unwanted flow in the channels was developed.