Dresden 2006 – wissenschaftliches Programm
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AKB: Biologische Physik
AKB 5: Cell Motility II
AKB 5.2: Vortrag
Montag, 27. März 2006, 14:30–14:45, ZEU 255
A biomimetic model system shedding light on active lamellipodial biomechanics — •Björn Stuhrmann1, Florian Huber1, Thomas Rudolph2, Klaus Zimmer2, and Josef Käs1 — 1Institute for Soft Matter Physics, University of Leipzig, Linnéstr. 5, 04103 Leipzig, Germany — 2Leibniz-Institut für Oberflächenmodifizierung e.V., Permoserstr. 15, 04303 Leipzig, Germany
In cells displaying crawling motility, cell boundary advancement is governed by the assembly of actin protein, tightly regulated by a wealth of accessory proteins. The key molecular players involved in these processes have been identified [1] and are used in this project to build a minimal model system of the cell lamellipodium. A polymerizing actin gel representing the lamellipodium is generated in nanostructured, cell-sized chambers. System confinement to cellular volumes is a crucial step towards cellular conditions and distinguishes our approach from existing assays. In the presence of ATP, the emerging gel represents for the first time a self-sustaining, polymerizing machine mimicking the cell lamellipodium in vitro. Offering the possibility to selectively change biochemical and physical parameters and to study the system‘s response in terms of its structural, dynamical and rheological properties, this model system presents a novel means to explore biomechanical mechanisms underlying cell motility.
[1] Loisel TP, Boujemaa R, Pantaloni D, Carlier MF. Nature. 1999 Oct 7;401(6753):613-6.