Dresden 2006 – wissenschaftliches Programm
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AKB: Biologische Physik
AKB 9: Chemical Bonds and Adsorption
AKB 9.2: Vortrag
Dienstag, 28. März 2006, 11:15–11:30, ZEU 260
Analysis of Adsorbed Protein Patterns at Surfaces — •Armin Nagel1, Hubert Mantz1, Christof Weitenberg1, Anthony Quinn2, Markus Bellion3, Ludger Santen3, and Karin Jacobs1 — 1FR 7.2 Experimental Physics - Soft Matter, Saarland University, D-66123 Saarbrücken — 2Department of Chemical and Biomolecular Engineering, University of Melbourne, Australia — 3FR 7.1 Theoretical Physics - Statistical Physics of Nonequilibrium and Disordered Systems, Saarland University, D-66123 Saarbrücken
The aim of this project is to identify the mechanisms for salivary protein adsorption at solid-liquid interfaces, with the intention of optimising the biocompatibility of dental replacement materials.
The methodology involves the characterisation of protein adsorption on surfaces that have been specifically tailored to have differing interaction potentials by varying the short and long range forces. The composition and topography of these tailored surfaces is carefully controlled and characterised via AFM and wettability analysis, enabling the structural and interfacial tension components to be identified.
AFM scans of the protein layers, both ex situ (in air) and in situ (in a buffer solution) are compared to ellipsometry measurements of the kinetics. The resulting complex patterns were analysed by image analysis methods (Minkowski Measures), so that tendencies during the adsorption process could be identified.
We compare the experimental protein patterns with patterns obtained by Monte-Carlo simulations of the protein adsorption process.