Regensburg 2007 – wissenschaftliches Programm

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CPP: Fachverband Chemische Physik und Polymerphysik

CPP 22: POSTER: INTERNAL SYMPOSIUM Optical Spectrosopy

CPP 22.3: Poster

Mittwoch, 28. März 2007, 16:00–18:30, Poster B

Fluorescence Lifetime Imaging Microscopy (FLIM) in the Frequency Domain — Wilhelm Hüttner and •Lothar Kador — Universität Bayreuth, Physikalisches Institut und Bayreuther Insitut für Makromolekülforschung (BIMF), 95440 Bayreuth

We report the design and construction of a confocal fluorescence lifetime imaging microscope (FLIM) based on a cw laser which is amplitude-modulated in the MHz range. Fluorescence lifetimes are calculated from the phase shift between the fluorescence signal and the excitation. The concept is inspired by a recent publication by Booth and Wilson [1] and allows one to choose different modulation frequencies between about 28 and 50 MHz. First experimental data are presented for blotting paper stained with a laser dye.

[1] M. J. Booth and T. Wilson, J. Microsc. 214, 36 (2004).