Regensburg 2007 – wissenschaftliches Programm
Bereiche | Tage | Auswahl | Suche | Downloads | Hilfe
CPP: Fachverband Chemische Physik und Polymerphysik
CPP 27: INTERNAL SYMPOSIUM Optical Spectroscopy II
CPP 27.3: Vortrag
Donnerstag, 29. März 2007, 15:15–15:30, H40
Proteins under pressure: spectroscopic determination of compressibilities — •Johannes Wiedersich1, Vladimir Ponkratov1, Mark Somoza1, Jane Vanderkoi2, and Josef Friedrich1 — 1Lehrstuhl für Physik Weihenstephan, Physikdepartment, TU München — 2Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, Pennsylvania
By means of optical spectroscopy we determine the local compressibility of proteins. At low temperatures the pressure shift of optical spectra can be precisely determined employing hole burning techniques. The values for the compressibility of ubiquitin and Zn horseradish peroxidase obtained at low temperatures are compared with those from high pressure experiments at ambient temperature. The results of both experiments show the same compressibility, indicating that the compressibility of proteins is the same whether the protein is surrounded by liquid at ambient conditions or whether it is frozen at low temperatures. In both cases the observed values are typical for solids.