Berlin 2008 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 15: Single Molecules
BP 15.1: Vortrag
Mittwoch, 27. Februar 2008, 14:00–14:15, C 243
Single molecule detection of Myosin V in living cell — •Paolo Pierobon1, Giovanni Cappello1, Sarra Achouri1, Sebastien Courty2, Maxime Dahan2, Alex Dunn3, and James Spudich3 — 1Institut Curie, Physico-Chimie-Curie, 11 rue P. et M. Curie, 75005 Paris, France — 2Laboratoire Kastler Brossel, Physics & Biology Department, Ecole Normale Supérieure 24, rue Lhomond 75005 Paris, — 3Dept. of Biochemistry, Stanford University School of Medicine, Beckman Center B405, Stanford , CA 94305
Single molecule imaging and manipulation provide an irreplaceable tool to isolate each component of the cell and to quantitatively study its dynamics in a perfectly controlled environment. However, the experiments are usually performed out of the physiological context and a priori no indication on the behaviour of the molecule in the cell can be given. To show that this limitation can be overcome, we marked with a quantum dots single myosin V (a processive motor whose physical properties have been largely investigated in vitro). We observed the motion of the motors at sub-pixel resolution directly in living cells. We measured for the first time the processivity, the speed and the step size of the motor in its natural environment and compared the results with the one obtained from in vitro experiments.