Berlin 2008 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 2: DNA and Chromatin
BP 2.6: Vortrag
Montag, 25. Februar 2008, 12:00–12:15, PC 203
Gold Nano-Stoves for Microsecond DNA Melting Analysis — •Calin Hrelescu1, Joachim Stehr1, Ralph A. Sperling2, Gunnar Raschke1, Michael Wunderlich3, Alfons Nichtl3, Dieter Heindl3, Konrad Kürzinger3, Wolfgang J. Parak2, Thomas A. Klar1, and Jochen Feldmann1 — 1Photonics and Optoelectronics Group, Physics Department and CeNS, Ludwig-Maximilians-Universität München, Amalienstr. 54, 80799 Munich, Germany — 2CeNS, Ludwig-Maximilians-Universität München, Amalienstr. 54, 80799 Munich, Germany and Fachbereich Physik, Philipps Universität Marburg, Renthof 7, 35037 Marburg, Germany — 3Roche Diagnostics GmbH, Nonnenwald 2, 82372 Penzberg, Germany.
In diagnostics, medicine and biophysics, the melting analysis of DNA is a very important tool. In current temperature ramp techniques the typical time scales for a DNA melting analysis range from several minutes up to one hour. Especially for high throughput DNA analysis a faster detection of the DNA melting point is highly desirable, as well as the successful identification of mutants of the target DNA. We exploit the characteristic plasmonic properties of DNA bound gold nanoparticle aggregates to optically induce and detect the melting of double stranded DNA. The aggregates are used as very efficient light absorbers to locally convert optical energy from laser pulses into thermal energy. Pulsed optical experiments show that heating on a microsecond timescale is sufficient to melt DNA molecules. Only one single laser pulse is needed to distinguish between a perfectly matching target and a target with a point mutation.