Berlin 2008 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 22: Cell Mechanics
BP 22.5: Vortrag
Donnerstag, 28. Februar 2008, 11:30–11:45, PC 203
The use of scanning probe techniques and laser micromanipulation to isolate and mechanostimulate highly potent adult mesenchymal stem cells — •Karla Müller1, Matthias Zscharnack2, Jörg Galle3, and Josef Käs1 — 1Inst. for Soft Matter Physics, University of Leipzig — 2Applied Stem Cell Biology, Center for Biotechnology and Biomedicine, University of Leipzig — 3Interdiciplinary Centre for Bioinfomatics, University of Leipzig
Degenerative joint diseases due to rheumatism, joint dysplasia or traumata are particularly widespread in countries with high life expectancies. Today hyaline cartilage and bone defects resulting from joint destruction can be treated by appropriate transplantations from (and thereby destroying) intact joint areas. An alternative approach is the use of adult mesenchymal stem cells. These cells have the potential to differentiate into various cell types, such as osteoblast-like cells and chondrocyte-like cells. The aim of MS CartPro is to develop a closed, aseptic bioreactor for the production of autologous grafts for cartilage regeneration. We establish the sorting of most potent cells out of a heterogeneous cell sample by exploring the phase space of viscoelastic properties and relating these to the individual cells ability to differentiate into the desired tissue type. In order to non-invasively probe the mechanical properties of suspended cells, the Optical Stretcher is a highly adequate tool. Mechanostimulation is achieved by indenting adherent stem cells with a modified AFM tip in order to push them towards a chondrocyte like differentiation.