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BP: Fachverband Biologische Physik
BP 26: Posters II
BP 26.20: Poster
Thursday, February 28, 2008, 17:00–19:30, Poster A
A robust surface plasmon resonance biosensor with high resolution — •Sebastian Horstmeier, Andy Sischka, Christoph Pelargus, Katja Tönsing, and Dario Anselmetti — Experimentelle Biophysik und Angewandte Nanowissenschaft, Fakultät für Physik, Universität Bielefeld, Universitätsstraße 25, 33615 Bielefeld
We present a simple, robust and easy to use surface plasmon resonance (SPR) biosensor with high resolution which is based on a previous study [1] and can be used in biophysical applications as well as for educational purposes, e.g. practical courses. SPR-based biosensors are able to investigate biochemical reactions according to their kinetics without labeling the molecules. Using the Kretschmann-Raether-configuration the SPR-angular shifts are detected by a method based on a quadrant photo diode like in atomic force microscopy (AFM). The goldfilm is divided into two areas, one for reference measurement, the other for analysing the specific binding-reaction. SPR-angular changes due to the bulk solution as well as errors caused by thermal drift, mechanical stress or fluctuations of the laser are eliminated by the reference measurement which is made parallel under the same conditions. Using this method changes of the refractive index in the order 10−8 refractive index units (RIU) can be measured. The experimental setup and first results will be presented and discussed.
[1] H.Q. Zhang, S. Boussaad and N.J. Tao: High-performance differential surface plasmon resonance sensor using quadrant cell photodetector, Review of Scientific Instruments, 74(1):150-153, 2002