Berlin 2008 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 4: Novel Methods

BP 4.4: Vortrag

Montag, 25. Februar 2008, 15:15–15:30, PC 203

Microscopical visualization of Gold-Nanoparticles for biological and medical applications — •Andrea Isabel Matschulat, Franz-Josef Schmitt, Max Schoengen, and Hans Joachim Eichler — Institut für Optik und Atomare Physik, Technische Universität Berlin, Strasse des 17.Juni 135, 10623 Berlin

The application of novel nanotechnologies, especially in biotechnology and nanomedicine looks very promising. Improved and partly new physical, chemical and biological properties of nanostructures make them to powerful tools in diagnostics and therapy. Gold-Nanoparticles in contact with living HCT-116 colon carcinoma cells were visualized with several microscopical techniques such as Scanning Electron Microscopy (SEM), Atomic Force Microscopy (AFM), Scanning Near-Field Optical Microscopy (SNOM) and conventional Light Microscopy (LM). VIS-Spectroscopy was applied for studying long-time-stability and optical properties of the Gold-Nanoparticles. The goal is the in vivo visualization of the nanoparticles in living cells. This project is treated in collaboration with Surgical Research Unit OP 2000, Max-Delbrück-Centrum für Molekulare Medizin, Charité - Berlin in vitro. Especially in the field of conventional optical Far-Field Microscopy, measurements with colloids in living cells using a dark-field configuration showed that resolution was limited in the size range of 1 µ m due to Abbe’s limit of resolution and scattering effects, however, the particles were identifiable through their red colour as a result of the plasmon resonance effect which was calculated with classical Drude-Lorentz-model.