Berlin 2008 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 7: Posters I
BP 7.12: Poster
Montag, 25. Februar 2008, 17:00–19:30, Poster A
Investigation of erythrocytes cell-cell adhesion forces using holographic optical tweezers — •Achim Jung1, Matthias Brust1, Patrick Steffen1, Christian Wagner1, Ingolf Bernhardt2, Ljubomira Ivanova2, Lars Kaestner3, and Peter Lipp3 — 1Department of Physics, Saarland University, 66041 Saarbrücken, Germany — 2Central Isotope Laboratory/Laboratory of Biophysics, Saarland University, 66041 Saarbrücken, Germany — 3Institute for Molecular Cell Biology, Saarland University, 66424 Homburg, Germany
Prostaglandin E2 (PGE2) and lysophosphatidic acid (LPA) are released from activated platelets. Using fluorescence imaging, spectral imaging and the patch-clamp technique, we recently provided evidence that these lipid-mediators at physiological concentrations activate a non-selective cation-channel in human red blood cells (RBCs). This results in a Ca2+ influx and the consecutive intracellular Ca2+ concentration increase. Ca2+ increases elicits the Ca2+-activated K+ channel (Gardos channel) in the RBC membrane resulting in K+ efflux and shrinkage of the cells. By means of holographic optical tweezers we investigate the inter-cellular adhesion forces between individual RBC arising after mediator stimulated increase of the intracellular Ca2+ concentration. We are going to compare this force to depletion controlled cell-cell adhesion forces in polymer solutions. Based on our results we conclude that the PGE2 and LPA responses of RBCs reveal a direct and active participation of these cells in blood clot formation.