Berlin 2008 – wissenschaftliches Programm
Bereiche | Tage | Auswahl | Suche | Downloads | Hilfe
BP: Fachverband Biologische Physik
BP 7: Posters I
BP 7.30: Poster
Montag, 25. Februar 2008, 17:00–19:30, Poster A
In vitro assembly and characterization of keratin intermediate filaments — •Anke Leitner1, Katrin Hübner1, Othmar Marti1, Harald Herrmann2, and Tatjana Wedig2 — 1Ulm University, Institute Of Experimental Physics, Germany — 2Division of Molecular Genetics, German Cancer Research Center Heidelberg, Germany
The aim of this work is to compare the properties of in vitro assembled keratin intermediate filaments with those of authentic keratin cytoskeletons. We will show the details of the assembly process of the keratin filaments. In a first step the recombinant keratin 8/18 dimers assemble into tetramers. In the second step the tetramers form unit length filaments (ULF). In a third step, the ULFs longitudinally anneal into loosely packed filaments and radially compact into mature intermediate filaments. The properties of the assembled filaments are investigated by means of atomic force microscope (AFM) and photonic force microscope (PFM) and are compared to those of cytoskeletons prepared from cultured human pancreatic cancer cells (line Panc 1).