Berlin 2008 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 7: Posters I
BP 7.6: Poster
Montag, 25. Februar 2008, 17:00–19:30, Poster A
Orientation - Defined Stretching and Fixing of DNA by AC Voltage Induced Electro-Osmotic Flow — •Venkatesh Alagarswamy Govindaraj1, Simone Herth1, Anke Becker2, Andreas Hütten1, and Günter Reiss1 — 1Thin Films and Nano Structures, Department of Physics, Bielefeld University, Bielefeld, Germany — 2Institute for Genome Research and Systems Biology, CeBiTec, Bielefeld University, Bielefeld, Germany
Application of DNA stretching at a single molecule level has become an attractive domain of research in the field of bioelectronics, genomics and nanobiotechnology. It was already reported that DNA could be stretched in an orientation-defined way between two electrodes with a gap of several micrometers using masks. In this work a new electrode design was fabricated to facilitate an orientation-defined stretching of dsDNA across a channel of submicron width. Tagged DNA strands of defined length were synthesized by Polymerase Chain Reaction using pUC19 as template. The forward and reverse primers were 5'-tagged with thiol and biotin, respectively. The tagged DNA strands were then dielectrophorized on modified interdigitated electrodes with oppositely placed castellation at an intermediate frequency in order to induce electro-osmotic flow. The stretched DNA was observed through laser confocal microscopy using intercalating dye such as acridine orange. This method enables characterization of single molecule dynamics and its consequent application in DNA based electronics, DNA templated nano structures, mutation detection and DNA-protein interaction studies.