Berlin 2008 – wissenschaftliches Programm
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CPP: Fachverband Chemische Physik und Polymerphysik
CPP 36: Biopolymers and Biological Systems
CPP 36.2: Vortrag
Freitag, 29. Februar 2008, 10:45–11:00, C 264
Digital in-line soft X-ray holography as microscopy technique for biological samples — •Axel Rosenhahn1, Ruth Barth1, Florian Staier1, Todd Simpson2, Silvia Mittler2, Stefan Eisebitt3, and Michael Grunze1 — 1Angewandte Physikalische Chemie, Universität Heidelberg, Germany — 2Department of Physics and Astronomy, The University of Western Ontario, Canada — 3BESSY m.b.H., 12489 Berlin, Germany
Digital in-line soft X-ray holography (DIXH) is a lenseless approach towards microscopy at short wavelength. We present measurements which successfully prove that the original Gabor geometry can be applied to X-rays and a lateral resolution of 400 nm can be achieved. Objects of different thickness and materials were used to determine the imaging properties of holographic microscopy in the VUV and soft X-ray wavelength range. By tuning the x-ray energy, element specific contrast can be obtained e.g. at the carbon K edge. These results are promising with respect to the possibility to exploit intrinsic contrast mechanisms for biological samples. Using fibroblasts and other cells, the sensitivity of the technique to resolve small structures inside these extended objects will be discussed. Although the spatial resolution still needs to be improved, these experiments are a starting point for future, lenseless holospectroscopy.