Berlin 2008 – wissenschaftliches Programm

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CPP: Fachverband Chemische Physik und Polymerphysik

CPP 36: Biopolymers and Biological Systems

CPP 36.3: Vortrag

Freitag, 29. Februar 2008, 11:00–11:15, C 264

Reptation of individual F-Actin Filaments in 3D Networks — •Masashi Degawa, Norbert Kirchgeßner, Bernd Hoffmann, Rudolf Merkel, and Margret Giesen — Institut für Bio- und Nanosysteme 4, Forschungszentrum Jülich, 52425 Jülich Germany

The mechanical properties of the cell are determined by a 3D network of polymerized protein filaments, the cytoskeleton. For deeper understanding of cell biomechanics, it is crucial to understand the physical properties of such polymer network. In the past, we have concentrated on the study of individual F-actin filaments within the network. Dynamics of the filaments were studied in the time scale of μsec to a sec where the transverse fluctuation from the filament contour is dominant and results were well described by the Glassy Worm-like chain Model [1]. Here we present dynamic studies of the same system, however, in the time scale of min to an hr where the motion along the filament contour is now dominant. F-Actin are labeled with TRITC and visualized with line-scan confocal scanning microscope. We measure the filament contour as a function of time. Specifically we focus on the curvature of the filaments, which in recent work indicate glass like properties, where the ergodic theorem breaks down [2]. [1] J. Glaser, et al. submitted to Eur. Phys. J. (2007) [2] M. Romanowska, et al. submitted to Phys. Rev. Lett. (2007)