Berlin 2008 – wissenschaftliches Programm

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CPP: Fachverband Chemische Physik und Polymerphysik

CPP 9: POSTERS Single Molecules, Biopolymers, Membranes

CPP 9.15: Poster

Montag, 25. Februar 2008, 16:45–19:00, Poster A

High energy reflectivity studies of protein adsorption on functionalized surfaces — •Kaveh Shokuie¹, Michael Paulus¹, Florian Evers¹, Federica Venturini², Sebastian Schoeder², and Metin Tolan¹ — ¹Experimentelle Physik Ia/ DELTA, Technische Universität Dortmund, Maria-Goeppert-Meyer Str. 2, 44221 Dortmund — ²European Synchrotron Radiation Facility, Grenoble, France

Adsorption of several proteins (Lysozyme, Hemoglobin, α-lactalbumin) on functionalized surfaces was investigated using high energy X-ray reflectivity. The main interest is the effect of different substrate surfaces on the protein adsorption behaviour (e.g. conformational changes of the protein). The applied technique, X-ray reflectivity, makes it possible to determine the sample’s density profile perpendicular to its surface with Angstrom resolution. The high energy reflectivities were taken at the ID15 beamline of the ESRF using 72.5 keV radiation. The high photon energy allows the in situ investigation of the protein adsorption at the solid-liquid interface.