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Berlin 2008 – scientific programme

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HL: Fachverband Halbleiterphysik

HL 17: Poster I

HL 17.4: Poster

Monday, February 25, 2008, 16:30–19:00, Poster D

Non-Oriented and Oriented Protein Immobilization on Diamond SurfacesSimon Quartus Lud1, •Philipp Sebastian Koch1, Florian Spirkl1, Rainer Jordan2, Paola Bruno3, Dieter M. Gruen3, Jose A. Garrido1, and Martin Stutzmann11Walter Schottky Institut, Technische Universität München, Am Coulombwall 3, 85748 Garching, Germany — 2Materials Science Department, Argonne National Laboratory, Argonne, Illinois 60439, USA — 3Chemistry Department, Technische Universität München, Lichtenbergstr. 4, 85748, Garching, Germany

Interfacial molecular assemblies play an important role for applications in all fields of biosensor design. Due to its numerous remarkable material properties, like its extreme stability, large electrochemical window and good biocompatibility, diamond is a very attractive candidate for chemical and biochemical sensing. We discuss various methods for the immobilization of proteins on a diamond substrate. More specifically, we report on random and oriented modes of binding to ultrananocrystalline diamond (UNCD) surfaces. Diamond thin films are first modified by pure chemical grafting of benzene diazonium salts with functional headgroups. AFM, XPS, electrochemical CV, and NEXAFS spectroscopy have been used to verify the direct covalent attachment of the aromatic molecules. The results confirm the presence of a very stable, homogeneous, and dense monolayer for both headgroups.

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