Berlin 2008 – wissenschaftliches Programm
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ST: Fachverband Strahlen- und Medizinphysik
ST 6: Heavy Ion Therapy and Related Basic Research II
ST 6.4: Vortrag
Dienstag, 26. Februar 2008, 12:15–12:30, H 2033
Fragment distributions of plasmid DNA analyzed by AFM - Local Effect Model for a freely jointed chain of monomers — •Thilo Elsässer1, Katarzyna Psonka2, Michael Scholz1, Ewa Gudowska-Nowak2, and Gisela Taucher-Scholz1 — 1Gesellschaft für Schwerionenforschung, Biophysics, Planckstr. 1, 64291 Darmstadt — 2Marian Smoluchowski Institute of Physics, Jagiellonian University,
The investigation of fragment length distributions of plasmid DNA gives insight into the influence of localized energy distribution on the induction of DNA damage, particularly the clustering of double strand breaks. We present an approach that determines fragment length distributions of plasmid DNA by using the Local Effect Model applied to a freely jointed chain of monomers as the traget model. We find a good agreement of our simulations with experimental fragment distributions derived from Atomic Force Microscopy (AFM) studies by including experimental constraints typical for AFM into the model calculations. The results of the model calculations can be used to determine the optimal parameters for future experiments. Additionally, we compare the results of our model for two different plasmid geometries.