Regensburg 2010 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 32: Posters: Physics of Cells
BP 32.19: Poster
Donnerstag, 25. März 2010, 17:15–20:00, Poster B1
Quantification of hematopoietic stem cell and neutrophil chemotaxis using microstructured systems and ELISA — •Christina Leinweber1, Rainer Saffrich2, Anthony D. Ho2, Nicole Niemeier3, Katja Schmitz3, Michael Grunze1,3, and Axel Rosenhahn1,3 — 1Applied Physical Chemistry, University of Heidelberg — 2Department of Medicine V, University of Heidelberg — 3IFG/ITG, Karlsruhe Institute of Technology
The migration of hematopoietic stem cells (HSC) towards bone marrow, the so called homing process, plays an important role in modern leukemia therapy. HSC are supposed to be guided by a concentration gradient of chemokines which are expressed by marrow cells, the mesenchymal stromal cells (MSC). Therefore we investigate the chemotactic response and migration behavior of HSC using different in vitro chemotaxis assays with increasing intricacy, e.g. migration experiments in microwells, transwells and within microstructured systems. These chip systems allow studying single parameters, such as migration kinetics, thresholds, sensing sensitivity and swarm behaviour, by varying the geometry of the microchannel structures. In order to establish the methods, particularly the microstructures, we also used neutrophil granulocytes differentiated from HL-60 cell line as a model system. Additionally we performed ELISA experiments to analyze the expression of the chemokine SDF-1 by MSCs, as SDF-1 is already known to be involved in the signalling process and most likely controls HSC migration. We determined the SDF-1 concentration in dependence on expression time and on MSC culture media.