Regensburg 2010 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 37: Biomaterials

BP 37.5: Vortrag

Freitag, 26. März 2010, 11:15–11:30, H43

Bioactive surfaces from polymeric films aiming at near IR-light triggered cellular response — •Dmitry Volodkin^1,3, Andre Skirtach¹, Narayanan Madaboosi¹, Jenifer Blacklock¹, Regine von Klitzing³, Andreas Lankenau², Claus Duschl², and Helmuth Möhwald¹ — ¹MPIKG, Am Mühlenberg 1, 14476 Potsdam-Golm, Germany — ²IBMT, Am Mühlenberg 13, 14476 Potsdam-Golm, Germany — ³TU Berlin, Strasse des 17. Juni 124, 10623 Berlin, Germany

The layer-by-layer (LbL) polymer self-assembly based on consecutive polymer adsorption has emerged as a powerful and versatile strategy to engineer surface films for bio-applications. Here we present composite LbL-assembled dynamic films possessing high loading capacity, remote release functionalities, and controlled cellular response. The film has been formed using two biopolymers, namely hyaluronic acid (HA) and poly-L-lysine (PLL). The film able to embed material of different nature (from flexible macromolecules such as DNA and proteins to nano- and microparticles) in extremely high amounts (tens of mkg per cm2) that is related to spontaneous "polymer doping". Here we present remote release of film-entrapped material by "biofriendly" near-infrared light. Composite HA/PLL film with embedded gold nanoparticles and biomacromolecules or microcapsules hosting biomolecules can be activated by infrared light resulting in biomolecules* release. The films can be constructed to be cell (fibroblasts) adhesive or cell resistant depending on its intrinsic properties. Light-triggered DNA transfection to individual cell is demonstrated.